COST-STSM FA0605-7452 XAVIER ZARZA
SCIENTIFIC REPORT
PhD student: XAVIER ZARZA
Host laboratory: Laboratory of systems biology and systems medicine,
University of Glasgow, UK
Date of stay: 9th - 15th January 2011
During my stay in the Laboratory of systems biology and systems
medicine (University of Glasgow) where I worked with Dr Anna Amtmann’s
team, I performed Chromatin Immunoprecipitation (ChIP) analysis of
shoot tissue harvested from Arabidopsis thaliana wildtype (Col0) for
specific histone methylation marks. The main aim of this stay was to
learn and optimize the protocol developed by Colot and colleagues for
ChIP-on-chip and adapt it for the identification of precipitated DNA-
sequences by Illumina sequencing (ChIP-Seq), in order to study the
possible involvement of several genes of polyamine catabolism pathway
in chromatin modifications.
The knowledge acquired in Glasgow will allow, in the near future, to
carry out the similar analysis in the Laboratory of Plant’s Physiology
at the Faculty of Pharmacy, University of Barcelona, Spain.