Kumud B Mishra

Company
Academy of the Sciences of the Czech Republic

Address
CzechGlobe - Global Change Research Center AS CR, v.v.i
136 Zamek
37333 Nove Hrady

Telephone
+420 776503246

E-mail
mishra@greentech.cz

1. Summary of work to be allocated into a specific WG

This partner is using non-invasive optical reporter signals (e.g., fluorescence and reflectance) of the plants using imaging and non-imaging techniques for probing regulations and detection of early stress responses. The well established fluorescence/reflectance parameters as well as parameters of advanced statistical approach will be used for the analysis of the data. The main interest is to co-operate with various groups in this consortium aiming to develop non-invasive reliable screening methods to enable to rapidly evaluate the various transgenic plants and its stress tolerance capacity that can be used for the high-throughput screening of stress resistance in plants. This method can be applied to all existing work group of the consortium. 

2. Links with other WG and/or Subgroups

Look forward to hear from the potential partners, in this consortium, who are interested to apply this advanced technique, to probe regulations of signaling/metabolic activity of the plants subjected to abiotic stress.

3.  Specific activities to be integrated into WG and/or Subgroups

WG1: Investigation of a relation between theSnRK1/2 kinases and fluorescence/ reflectance parameters under stated stress.

WG2 Study the sensitivity of the fluorescence/reflectance parametersto screen the alteration of the ABA signaling pathways under the stated stress conditions.  

WG3: Identification of fluorescence/reflectance parameters sensitive to the praline accumulations.

WG4: Investigation of the set of parameters resulting from fluorescence/reflectance signals to probe the polyamine mediated changes under abiotic stress.

4. Qualification for the role and current grants

Kumud B. Mishra is a scientist currently associated with the Institute of Systems Biology and Ecology (Nove Hrady), Academy of Sciences in Czech Republic. He has more than six years of experience in studying the plants response subjected to abiotic stresses using non-invasive spectroscopic imaging and non-imaging techniques. The work is currently financed by a grant approved by Ministry of Czech Education, Czech Republic.

5. Technical expertise and facilities

This partner will provide all of the experience required for the in-vivo measurement of fluorescence (Kinetic imaging fluorometer) and reflectance (spectrometer for visible/UV range) signals of the plants. Recently developed HPLC methods using c-30 column for the quantification of photosynthetic pigments (special attention to xanthophylls cycle) will be provided. The instruments (gas exchange system, spectrophotometry, spectrofluorometry, microscope etc. ) developed jointly by Institute of System Biology and Ecology and Institute of Physical Biology, Czech Republic can be provided .